Pre-formulated shoot-stage media for consistent genetic multiplication — just add water.
Athena Shoots Culture Media (TC-CM-ST-125) is a dry-sachet, all-in-one shoot-stage tissue culture formula engineered for cannabis and hemp micropropagation workflows. Dissolve in RO water, autoclave, and pour — no weighing, no custom blending, no guesswork.
Athena Shoots Culture Media is a pre-formulated, dry-powder sachet designed specifically for the shoot-multiplication stage of plant tissue culture. Each 125 mL sachet contains a complete all-in-one nutrient and media formula — no separate agar weighing, no cytokinin or auxin measuring required. Dissolve the sachet contents in RO water to the 125 mL fill line, shake to dissolve, sterilize in an autoclave, then pour into culture vessels before inserting plant material.
The Shoots formula is the Stage II media in Athena's Culture Line workflow, purpose-built to support vigorous new shoot development and genetic replication from established explants. It is also specified by Athena as usable for seedling-start applications within sterile workflows. A companion Roots Culture Media formula handles Stage III rooting once your shoot material is ready to transition.
Any licensed cannabis or hemp operator running an in-house tissue culture program — whether a multi-site MSO centralizing genetic preservation, a single-site cultivator building a clean mother library, or a propagation specialist managing cultivar integrity across runs. Shoots Culture Media is the consumable that keeps your TC program running between kit restocks.
This product is particularly relevant for operations dealing with hop latent viroid (HLV) pressure. Athena's Culture Line protocol is designed to support workflows that reinvigorate genetics and reduce contamination risk by working entirely within a sterile, in vitro environment — limiting the mechanical transmission vectors that spread HLV through conventional cutting programs. If your propagation room has had an HLV event, a properly run TC program using sterile shoot-stage media is one of the few evidence-backed paths to clean stock multiplication.
The all-in-one dry-sachet format eliminates the formulation errors that come with weighing out individual media components. Each sachet is sealed for freshness and long-term stability — useful for operations that run TC on a campaign basis rather than continuously. The format is also well suited for bulk procurement: sachets pack at 10 per box, 12 boxes per case, making it straightforward to stock adequate consumables for multi-vessel production cycles.
Shoots Media is engineered to encourage new shoot growth and support genetic replication from existing explant material. Sterility depends on workflow: all steps — mixing, pouring, and inserting plant material — should be performed under an appropriate laminar flow hood, and media must be sterilized in an autoclave before use. The Athena Culture Line ecosystem includes a portable Flow Hood and purpose-built Autoclave for operators who need a complete bench-top setup.
Shoots Culture Media is a refill consumable for any operator already running the Athena Culture Line system, or any operation with an existing flow hood and autoclave setup that wants a standardized, pre-formulated media rather than a custom in-house blend. The sachet format removes per-batch formulation variability — a common source of failed vessels in commercial TC programs.
For operations building out a new TC propagation workflow, Shoots Media pairs directly with Athena's Roots Culture Media (Stage III), Culture Vessels, Utility Vessels, and the Culture Kit system. HSC carries the full Athena Culture Line; reach out for case-quantity pricing and to spec the right media volumes for your target vessel output.
| SKU | TC-CM-ST-125 |
| Brand | Athena Ag |
| Product Line | Athena Culture Line |
| TC Stage | Stage II — Shoot Multiplication |
| Format | Dry-powder sachet; dissolve in RO water |
| Sachet Yield | 125 mL per sachet |
| Pack Size | 10 sachets per box |
| Case Pack | 12 boxes per case |
| Water Requirement | RO water to 125 mL fill line |
| Sterilization Method | Autoclave required prior to use |
| Also Available In | 750 mL sachet size |
Shoots Media is formulated for Stage II — shoot multiplication — where the goal is generating new shoot growth and replicating genetic material from an established explant. Roots Media handles Stage III, supporting root formation and plantlet development once shoot material is ready to transition. Most TC programs use both in sequence.
No. Shoots Media is a standalone consumable. If you already have a laminar flow hood and autoclave — whether Athena's purpose-built units or lab-grade equivalents — you can run Shoots Media in your existing setup. All steps (mixing, pouring, plant insertion) must be performed in a sterile airflow environment.
Athena's Culture Line protocol is specifically designed to support workflows aimed at outgrowing HLV infections and preserving clean genetics in a sterile in vitro environment. Shoots Media is the Stage II consumable within that workflow. It does not itself 'cure' HLV — the sterile propagation process is what limits mechanical transmission and allows clean shoot material to be multiplied. Consult Athena's documented TC procedure for the full protocol.
Each 125 mL sachet yields 125 mL of prepared media. The number of vessels depends on your vessel size and fill volume. Athena's Culture Kit (which includes Shoots and Roots media) is rated to produce up to 120 culture vessels from its included media supply across both stages. Scale your sachet order to your target vessel output.
Yes. Shoots Culture Media is also available in 750 mL sachets, also packed 10 sachets per box. Contact HSC to discuss which sachet size and case quantity fits your production volume.
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